摘要
本文以百脉根子叶外植体为受体材料，通过根癌农杆菌介导的方法，导入外源目的基因1－磷酸甘露醇脱氨酶（mtlD）基因和npt－Ⅱ基因，建立了转化系统，获得转基因植株，转化频率为9．7％．转化处理的外植体再生植株的茎段在含卡那霉素的分化培养基上膨大，再生成苗和植株。抗性植株的形态正常，移入盆栽后生长良好。PCR扩增与扩增产物的Southern杂交证明外源基因已整合到百脉根基因组上。
Transgenic plants of Lotus corniculatus L. were obtained by Agrobacterium - mediated transformation. utilizing a disarmed Ti plasmid pBin438 which containing mannitol-1-phosphate dehydrogenase gene mtlD and kanamycin resistance gene npt-II. The transformation frequency was 9. 7%. Plants were regenerated from segments of kanamycin-resistant plants on kanamycin containing medium. Plants were morphologically normal and grew well after being transfered to pot. The results of PCR amplification and Southern blot hybridization confirmed that mtlD and npt-II gene were integrated into the Lotus corniculatus L. genome.
出处
《草地学报》
CAS
CSCD
1996年第1期7-11,共5页
Acta Agrestia Sinica
基金
国家科委863计划
关键词
百脉根
根癌农杆菌
基因转化
再生植株
Lotus corniculatus L.
Gene transformation
Plant regeneration
Agrobacterium tumefaciens
