摘要
目的建立血液、脑脊液中尼可刹米的HPLC检测方法.方法取空白血液或脑脊液添加不同量的尼可刹米对照液,碱化后用二氯甲烷等有机溶剂提取,用HPLC方法检测.对方法的萃取回收率、线性、精密度等进行考察.结果方法选用二氯甲烷作为萃取溶剂,萃取回收率大于60%.血液中尼可刹米的在0.5～100μg/ml范围内线性良好,R2为0.9993;脑脊液中尼可刹米的线性范围为0.1～100μg/ml,R2为0.9996,最低检出限是5ng(S/N≥3).检测血液和脑脊液中的尼可刹米方法的精密度均小于6%.结论所建方法准确、简便、灵敏度高,适用于法医毒物分析和临床药物分析.
Objective To develop a high-performance liquid chromatographic method for determination of nikethamide in human blood and cerebrospinal fluid. Methods The compounds was extracted from blood or cerebrospinal fluid by liquid-liquid extraction using dichloromethane after blood or cerebrospinal fluid was adjusted pH. The extracts were analyzed by HPLC. Results Linear limits of nikethamide determination in blood ranged from 0.5μg/ml to 100μg/ml （R^2 = 0. 9993）. Linear limits of nikethamide determination in cerebrospinal fluid ranged from 0.1μg/ml to 100μg/ml （R^2 = 0. 9996）. Limit of detection was 5ng（S/N≥3）. The extraction recovery was over 60 % and its coefficients of variation were less than 6 %. Conclusion The method is sensitive, simple, rapid and suitable for determination of nikethamide in human blood and cerebrospinal fluid for toxicological and clinical pharmaceutical analysis.
出处
《刑事技术》
2005年第5期31-33,共3页
Forensic Science and Technology
