四倍体百合体细胞形态发生中三种同工酶谱变化的研究

STUDIES ON THE CHANGES OF THREE KINDS OF ISOENZYME ATLAS IN MORPHOGENESIS OF SOMATIC CELLS OF TOTETRAPLOID LILY

首次报道了四倍体龙牙百合体细胞形态发生的激素调控及不同形态发生类型中酯酶、淀粉酶与过氧化物酶同工酶谱动态变化的研究结果。ＭＳ培养基中分别添加６－ＢＡＩ～２ｍｇ／Ｌ和２，４－Ｄ２ｍｇ／Ｌ，四倍体龙牙百合鳞片叶外植体则分别按器官型和器官发生型两种途径进行形态发生；分析上述３种酶同工酶谱，发现它们分别反映出其形态发生的进程，但在两种不同途径中存在明显差异，主要表现器官发生型形态发生其细胞内同工酶经历了比器官型形态发生更为深刻的变化，表明这３种酶同工酶谱的变化与形态发生明显相关，可以作为形态发生进程及途径确定的重要生化指标。

This paper reported,the first time,the results about the hormone regulation ofmorphogenesis of totetrapeoid lily“Nongya Baihe”and the isoenzyme altas changes of es-terase, amylase and peroxidase in different type morphogenesis during the cuIture of theplant.Organ-type inorphogenesis brought about when scale leaves were cultured on mediumwith 1～2mg/L 6-BA or organogeny-type morphogenesis brought about when cultured onmedium with 2 mg/L 2,4-D.. The changes of isoenzyme atlas of the three enzvmes men-tioned above reflected respectively the process of morphogenesis,but there were obvious dif-ferences in two different pathways of morphogenesis.The changes of isoenzyme atlas inorganogeny-type morphogenesis were greater than in organ-type morphogenesis. These re-sultes indicated that those three isoenzymes had obvious relationship with morphogenesis. Ihey may be used as useful and important biochemical marks to decide the process and path-way of morphogenesis.