体外成熟猪卵母细胞的体外受精研究

STUDIES ON THE IN VITRO FERTILIZATION OF PIGS

实验采用体外成熟的猪卵巢卵母细胞，将鲜精以前培养结合咖非因法获能后进行ＩＶＦ实验．体外成熟卵ＩＶＦ后的卵裂率为２４．７％～３８．４％，与目前文献报道的近似；授精时精子浓度为２×１０５／ｍＬ，１×１０６／ｍＬ和５×１０６／ｍＬ体外受精后的卵裂率分别为４０．８％（８２／２０１）．４５％（８５／１８９）和３０．５％（５０／１６４）．前二者无显著差异，但都与最后一组差异显著．我们认为，在本实验条件下，体外受精时适当的精子浓度要比目前常用的１×１０６／ｍＬ低．我们将肝素结合咖啡因的精子在能方法与前培养结合咖啡因法进行了比较，卵裂率分别为１０．７％（９／８４）和４０％（３２／８０），前者虽然在牛体受精实验中很成功．但在猪效果差．将体外成熟体外受精卵移入同步的受体猪输卵管后．有一头母猪成功地产下了５只“试管猪’，存活３只．

The cleavage rates(24.7%～38.9%) of the oocytes matured and fertilizedin vitro in this study were similar to those reported recently.The cleavage rates afrer IVF with 2×105 sperm/mL and 1×106 sperm/mL were not different significantly(40.8%vs.45%),but both differed remarkably from thosc obtained with 5×106 sperm/mL(30.5%). The acrosome reaction was induced in many spermatiozoa capacitated by this method was much lower but the cleavage rate after IVF with spermatozoa capacitated by this method was much lower than that by the capacitation method of prcincubation combined with caffeine(10.7% vs.40%).Some of the eggs after IVF were transrered surgically to the ovlducts of nine synchronized gilts, 35～72 each, and 3 pregnancies were comfirmed by zinc sulfate test one month after transfer. Five living piglets were obtained from one pregnant.