微量元素对超氧化物歧化酶活性的影响

The Eaffect of Se,Mg,F-in Different Concentrations on SOD Activity

用ＮＢＴ－Ｐ法、火焰原子吸收法，测定了不同浓度Ｓｅ、Ｍｇ、Ｆ－条件下ＳＯＤ标准品的活性和反应体积中Ｓｅ、Ｍｇ、Ｆ－含量。结果显示：（１）Ｓｅ浓度为７．５μｇ／Ｌ，ＳＯＤ活性达５９．００ｍｇ／Ｌ；Ｓｅ浓度由０．００μｇ／Ｌ增加至７．５μｇ／Ｌ时，ＳＯＤ活性由４２．５ｍｇ／Ｌ升至５９．００ｍｇ／Ｌ；Ｓｅ浓度继续增加到１０．００μｇ／Ｌ、２０．００μｇ／Ｌ时，ＳＯＤ活性均降为４２．５ｍｇ／Ｌ。（２）Ｍｇ浓度为３０．００ｍｇ／Ｌ时，ＳＯＤ活性由９６．５ｍｇ／Ｌ，Ｍｇ浓度由０．００ｍｇ／Ｌ增至３０．００ｍｇ／Ｌ，ＳＯＤ活性达４２．５ｍｇ／Ｌ，升至９６．５ｍｇ／Ｌ；继续增加Ｍｇ浓度到５０．００ｍｇ／Ｌ，ＳＯＤ活性降为７５．５ｍｇ／Ｌ。（３）Ｆ－由０．００ｍｇ／Ｌ增加到５．００ｍｇ／Ｌ时，ＳＯＤ活性由４２．５ｍｇ／Ｌ升到４９．５ｍｇ／Ｌ；继续增加Ｆ浓度到１０．００ｍｇ／Ｌ，ＳＯＤ活性降为４４．５ｍｇ／Ｌ；再增加Ｆ浓度至２０．００ｍｇ／Ｌ、４５．００ｍｇ／Ｌ后，ＳＯＤ活性维持在４９．５～５１．０ｍｇ／Ｌ之间。提示：ＳＯＤ活性受微量元素Ｓｅ、Ｍｇ、Ｆ－浓度的影响较大，只有在最适微量元素浓度条件下，ＳＯＤ活性方可达最高。

Adopting NBT-Pand flame atomic ab-sorption methods,the activity of SOD sample by differ-ent concentrations of Se,Mg,F in different pH,tem-peratures,times were measured.The results showed:(1)Se affects SOD activity:when concentration of Serernains 0.00μg/L,SOD activity appears 42.5mg/L;concentration of Se 7.5μg/L, SOD activity up to59.0mg/L;concentration of Se 10.0μg/L,SOD activitydown to 42.5mg/L;concentration of Se up to20.0μg/L,SOD activity remains 42.5mg/L;concentra-tion of Se up to 20.0μg/L, SOD activity remains42. 5mg/L.(2) Mg affects SOD activity:when concen-tration of Mg remains 0.00mg/L,SOD actlvity appears42.5mg/L;concentration of Mg up to 30.00mg/L,SOD activity up to 96.5mg/L; concentration of Mg upto 50. 00Mg/L,SOD activity down to 75.5mg/L.(3)F affects SOD activity:when concentration of F re-mains 0.00mg/L,SOD activity appears 42. 5mg/L;concentration of F up to 5.00mg/L, SOD activity up to49.5mg/L;concentration of F-up to 10.00mg/L,SODactivity down to 44. 5mg/L;concentration of F-up to20. 00mg/L,SOD activity back to 49 5mg/L;concen-tration of F up to 45.0mg/L,SOD activity appears49.5～51. 0mg/L.It suggests that SOD activity ap-pears much affected by the concentration of trace ele-ments and several physical factors,only when the conec-ntration of trace elements in the fittest conditions,SODactivity can reach up to the most.