颈淋巴结转移动物模型的建立及其微循环特性研究

Establishmentofcervicallymphnodemetastasisinmodelratsanditsmicrocirculationcharacteristics

为探讨血压变化对颈淋巴结转移瘤组织血流的影响，建立了颈淋巴结转移的动物模型，并测定了不同血压状态下转移瘤组织血流。给２０只雄性Ｄｏｎｒｙｕ大鼠耳廓背侧移植ＡＨ１０９Ａ癌细胞１４天后，使用氢气清除法测定不同平均动脉压状态下的组织血流量。结果２０只大鼠中有１７只发生同侧颈淋巴结转移。其肝、脑及骨髓的血流量当升压至２０ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ）左右或降压至９．０６ｋＰａ左右时均无明显变化（Ｐ＞０．１）；而颈淋巴结转移灶的组织血流量在正常血压（１４．８１±１．０４ｋＰａ）时为１．４２±０．４８ｍｌ·ｋｇ－１／ｓ（ｎ＝１３），升压状态（１８．９３±０．９２ｋＰａ）下血流量增多至３．４５±１．３３ｍｌ·ｋｇ－１／ｓ（ｎ＝１３，Ｐ＜０．０５），降压状态（８．８４±０．６３ｋＰａ）下血流量骤然减少至０．５５±０．２５ｍｌ·ｋｇ－１／ｓ（ｎ＝１３，Ｐ＜０．０５），近乎于血流停止。结论：颈淋巴结转移瘤组织血流可随血压变化增多或减少。依此可望提高化疗效果。

Todeterminetheefectsofvasoactiveagentsonbloodflowinlymphnodemetas-tases,thebloodflowinthecervicallymphnodemetastasesandnormaltissueweremeassuredusingahydrogenclearancemethod,atnormotensivestate,angiotensinⅡ(AⅡ)inducedhypertensivestateandsodiumnitroprusside(SNP)inducedhypotensivestate.Themetastasesoccurredfrequentlyinthecervicallymphnodesof17ratsonday11～14afterYoshidaasciteshepatomaAH109Acelsweresubcutaneouslyinoculatedintobackofauriculeof20rats.Thebloodflowintheliver,brainandbonemarrowofnormalratswasofnosignificantchangeswhenthemeanarterialbloodpressure(MABP)waselevatedtoapproximately20kPabyinfusionofAⅡanddecreasedtoapproximately9.06kPabyinfusionofSNP.Butthebloodflowinthemetastaticlymphnodeswasincreasedfrom1.42±0.48ml·kg-1/sto3.45±1.33ml·kg-1/s(n=13,P<0.05)whentheMABPwaselevatedfrom14.81±1.04kPato18.93±0.92kPabyAⅡ.Onthecontrary,thebloodflowinthemetastaticlymphnodewasdecreasedto0.55±0.25ml·kg-1/s(n=13,P<0.05)whentheMABPwasde-creasedto8.84±0.63kPabySNP.Theresultsindicatedthatmicrocirculationcharacteristicsinthecervicallymphnodemetastaseswerediferentfromthoseinthenormaltissues.AⅡinducedhyper-tensionwasusefultoselectivelyimprovedeliveryofchemotherapeuticdrugstothesiteofmetas-tases.Ontheotherhand,SNPinducedhypotensionmaybeusefultosuppresswashoutofthedrugs.