甲状腺功能异常患者末梢血淋巴细胞T_3核受体基因表达

EXPRESSIONOFT3RECEPTORGENESINPERIPHERALLYMPHOCYTESFROMPATIENTSWITHTHYROIDDYSFUNCTION

为了解甲状腺功能异常患者末梢血淋巴细胞Ｔ３核受体基因表达情况，以人ｃ－ｅｒｂＡα和ｃ－ｅｒｂＡβ的ｃＤＮＡ片段为探针，应用分子杂交技术，检测了５例Ｇｒａｖｅｓ病和７例桥本甲状腺炎伴甲状腺功能减退患者末梢血淋巴细胞ｃ－ｅｒｂＡα和ｃ－ｅｒｂＡβｍＲＮＡ的相对含量。结果表明：人淋巴细胞ｃ－ｅｒｂＡαｍＲＮＡ有６．０ｋｂ和３．２ｋｂ两种形式，而ｃ－ｅｒｂＡβｍＲＮＡ有５．０、３．０和２．０ｋｂ三种形式。桥本甲状腺炎伴甲状腺功能减退患者末梢血淋巴细胞ｃ－ｅｒｂＡα和ｃ－ｅｒｂＡβｍＲＮＡ表达增强，但Ｇｒａｖｅｓ病患者无明显变化。提示：桥本甲状腺炎伴甲状腺功能减退患者血清甲状腺激素水平减低，对其末梢血淋巴细胞Ｔ３核受体亚型在基因转录水平有向上调节作用。

InordertoexploretheexpresionandregulationofT3receptorgenes,therelativeamountofperipherallymphocytesc-erbAαandc-erbAβmRNAfrompatientswithGravesdiseaseandHashimotothyroiditiswithhypothyroidism(HTH)wasdeterminedwithhybridizationbyusinghumanc-erbAαandc-erbAβprobes.Theresultsshowedthatthereweretwotypesofc-erbAαmRNA(6.0kb,3.2kb)andthreetypesofc-erbAβmRNA(5.0kb,3.0kb,2.0kb).Thelevelsofc-erbAαandc-erbAβmRNAwerefoundtoincreaseinHTHpatients,butunalteredinGravesdiseasepatients.Thisresultsuggeststhepres-enceofup-regulationofnuclearT3receptoratthegenelevelinthelymphocytesofhypothyroidpatients.Thehighlevelofc-erbAαandc-erbAβmRNAmaycontributeinparttotheincreaseinT3receptor.