Ge—132对人乳癌细胞株BCaP—37的生物学作用

Biological Effects of Ge 132 on Chinese Breast Cancer BCaP-37 Cell Line

应用细胞培养技术，分子杂交的Ｎｏｒｔｈｅｒｎ Ｂｌｏｔ法和图像细胞分析技术（ＩＣＭ），研究Ｇｅ—１３２对人乳癌细胞株ＢＣａＰ—３７的生物学作用。细胞生长曲线的结果显示，Ｇｅ—１３２的３个实验组（１０．０ｍｇ／Ｌ，１００．０ｍｇ／Ｌ，２００．０ｍｇ／Ｌ）对细胞的生长没有显著抑制作用，并对细胞的毒性作用很小。基因检测显示，高浓度Ｇｅ—１３２（２００．０ｍｇ／Ｌ）对癌基因Ｃ—ｅｒｂＢ，和癌相关基因ＥＧＦＲ ｍＲＮＡ的表达具有抑制作用。ＩＣＭ的四项指标显示，Ｂ和Ｃ两实验组（１０．０ｍｇ／Ｌ，２００．０ｍｇ／Ｌ）ＤＩ值呈异倍体。表现为恶性细胞的特征，两组核面积显著低于对照组（Ｐ＜０．０ｌ）：Ｃ组的Ｓ期百分比显著低于对照组（Ｐ＜０．０５）。综合评价ＩＣＭ各项指标发现，Ｇｅ—１３２（２００．０ｍｇ／Ｌ）对细胞的增殖有一定抑制作用。

Biological effects of Ge-132 on Chinese breast cancer cell line--BCap-37 were studied by nieans of cell culture. Northern Blot and Iniage Cytonietry(ICM).Cell growth curves,showed that Ge-132(10.0,100.0,200.0mg/L)did not inhibit significantly the cell growth,and the toxicity of Ge-l32 to the cell was very low,The results of genc expresion indicated that Ge-132 in high concentration(200.0mg/L)inhibited C-erbB2 and EGFR mRNA expression, Four items were tested by ICM,DI in B and C groups showed the feature of malignant tumor cells. Nucleus areas in two groups(l0.0mg/L.,200. 0mg/L)were signifi-cantly lower than that in control group,and S%in C group(200.0mg/L)was significantly lower.The results of ICM suggestd that Ge-l32 inhibited significantly BCaP-37 cell proliferation by the general evaluation.