耳蜗活体铺片术

The cochlea in short-term culture

为了进行耳蜗器官培养，研究了小鼠耳蜗活体铺片标本的制作方法及其形态特征。用微分干涉相衬和双重荧光活体显微检查法，观察到短期培养的耳蜗活体铺片中毛细胞可保持良好活性达8～18小时。在1mmol／L双氢链霉素作用下，毛细胞在0．5～4．5小时开始变性，10～20余小时后全部死亡。耳蜗活体铺片术将成为耳蜗器官培养和细胞分子神经生物学研究中的重要手段。

In order to establish organotypic culture of the coch1ea, the dissection of the early postnatal mouse cochlea and its morphology in short-term culture were investigated. The viability of hair ceIls in the culture was assessed using differential lnterference contrast (DIC) microscopy and double-staining with fluorescein diacetate (FDA) and propidium iodide (PI). Tbe hair cells remainedviable and did not show any signs of damage for up to 8~18 hours. When incubated in Hank's solu-tion containing 1 mmol/L dihydrostreptomycin, the hair cells initially deteriorated in 0. 5~4. 5 hours and progressed to entire degeneration after 10~ 20 hours. In addition to its utilization in organotypic culture, the cochlea in short-term culture can be used as a valuable model for studying cellular and molecular neurobiology of the inner ear.