不同品种鱼腥草中槲皮素含量的检测与比较

Determination and Comparison of the Content of Quercetin in Different Kinds of Houttuynia Cordatas Thunb

建立鱼腥草中槲皮素的测定方法。90%乙醇为溶剂,索氏提取器提取,HPLC测定槲皮素的含量。Dia-monsilTMC18(4.6mm×150mm,5μm)为色谱柱,流动相为CH3OH-H2O-HAc(48∶49∶3),检测波长为254nm。槲皮素线性范围0.00704—0.06336μg,平均回收率和RSD分别为98.9%和3.2%。方法简便、准确、重现性好,可供鱼腥草原料选择和制剂质量控制的参考。

A new HPLC method for the determination of quercetin in Houttuynia cordatas thunb was established.90% EtOH was used as solvent and soxtherm distill machine was used to distill the quercetin.Diamonsil~ TMC_ 18(4.6mm×150mm.5μm) and MeOH-H_2O-HAc(48∶49∶3) was made use of chromatogram colum,mobile phase,respectively.The detection wavelength of quercetin is 254nm.The lineal range of quercetin is 0.00704—0.06336μg and the average recovery rate is 98.3% and RSD is 3.2%.The method is simple,sensitive and reproducible,which can be used for the qulity control of Houttuynia thunb cordata material and preparation.