摘要
目的：研究葡萄胎的ＤＮＡ来源及其与临床转归的关系。方法：４１例葡萄胎标本主要采自北京地区，以吸宫术后８周血ｈＣＧ高于正常为恶变标准。用Ｊｅｆｆｒｅｙｓ探针３３．１５与这４１例葡萄胎标本及夫妇双方血ＤＮＡ的ＨｉｎｆＩ限制性片段作Ｓｏｕｔｈｅｒｎ吸印杂交，获得ＤＮＡ指纹，经过对比分析，判定葡萄胎的ＤＮＡ来源。结果：３２例标本获得了清晰的ＤＮＡ指纹图谱，其中ＤＮＡ完全来自父源者２１例，恶变６例，占２８．６％（６／２１）；ＤＮＡ来自双亲者１１例，均无恶变。结论：ＤＮＡ完全来自父源与来自双亲的葡萄胎相比较，其恶变风险的差异有显著性（Ｐ＜０．０５）。
Objective:Tostudythegeneticoriginofhydatidiformmoles(HM)andtheclinicaloutcome.Methods:41casesofhydatidiformmolewerecolectedmainlyfromBeijingareaandstudiedforgeneticcomponentsbyDNAfingerprints.Thedignosisofpersistentgestationaltrophoblasticdisease(PTD)wasmadeifbloodhCGremainedhigherthannormal8weeksaftermoleevacuation.Results:Among41HMsamples,32producedidentifiableDNAfingerprints.DNAfromonlypaternaloriginwasfoundin21,inwhich6werePTD,DNAfrombothparentsin11,noneofwhichdevelopedPTD.Conclusions:DNAfromonlypaternaloriginwasmorefrequentlyfoundthanfrombothparentsinHM,andtherewasahigherriskofPTDintheformergroupthaninthelatter.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1996年第4期203-205,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
葡萄胎
DNA
指纹法
病理学
HydatidiformmoleDNAfingerprintingPathology
