小儿恶性淋巴瘤的细胞遗传学研究

Acytogeneticalstudyonchildhoodmalignantlymphoma

为寻找小儿恶性淋巴瘤（ＭＬ）染色体改变的规律及与其病理组织学的相关性，并探讨染色体重排与ＭＬ的关系，应用淋巴结改良直接法、短期培养法及骨髓短期培养法对２４例小儿ＭＬ进行细胞遗传学研究，２３例获得满意分裂相。结果表明：全部淋巴结染色体及４例骨髓染色体显示克隆性异常。非霍奇金淋巴瘤（ＮＨＬ）染色体众数多为二倍体及亚二倍体，分布似与组织学分型有关；霍奇金淋巴瘤（ＨＤ）染色体众数从亚二倍体到四倍体。染色体结构异常以６ｑ－较多见。本组出现的染色体相互易位大都涉及到１４ｑ３２及其附近，表明１４ｑ３２的断裂重排与ＭＬ的发生有关。

Inordertodiscovertheregularityofchromosomeabnormalitiesinmalignantlym-phoma(ML)anditsrelevancetohistopatholoigy,andtostudytherelationshipbetweentheoccur-renceofMLandchromosomeabnormalities,weearriedoutacytogeneticalstudyof24childrenwithML.Outofthe24cases,mitoticcelsthatcouldbeusedforanalysiswereobtainedin23.Theresultshowedthattheclonalabnormalitieswerefoundinlymphnodechromosomesofalthecasesandinthebonemarrowchromosomesof4cases.Incasesofnon-Hodgkin′slymphoma(NHL),themodalnumberofchromosomeswasmostlydiploidorhyperdiploidandthedistributionappearedtobecorre-latedwiththetypeofML.Ontheotherhand,inHodgkin′sdiseasesthemodalnumberofchromo-somesvariedfrombiploidtofourploid.Thecommonabnormalchromosomestructurewas6q-andthereciprocaltranslocationsofchromosomeweremostlyinvolvedinthe14q32ornearit.Theresultsuggeststhattheaberrationandrearrangementof14q32beassociatedwiththedevelopmentofML.