胚胎隔或隔与蓝斑组织联合移植治疗老年性痴呆动物模型的形态学研究

MORPHOLOGIC STUDY OF THE ANIMAL MODEL OF SENILE DEMENTIA TREATED WITH FETAL SEPTAL OR COMBINED SEPTAL WITH LOCUS CERULEUS TISSUE

以单侧横断老年大鼠穹窿伞的方法建立老年性痴呆模型，并将胚胎隔或隔与蓝斑联合移植物悬液注入模型鼠的双侧海马；１６周后用胆碱乙酰转移酶（ＣｈＡＴ）及酪氨酸羟化酶（ＴＨ）免疫细胞化学法观察移植物的存活、生长及与宿主海马的关系；用乙酰胆碱酯酶组织化学染色法观察宿主海马内胆碱能纤维的分布并定量ＣＡ１、ＣＡ３及齿状回中的纤维密度。结果表明：隔及联合移植物均能存活，但隔移植物生长体积明显大于联合移植物，移植物中ＣｈＡＴ阳性神经元丰富，多分布于团块中央及与宿主海马交界处，偶见ＴＨ阳性神经元分散分布。损伤侧海马ＣＡ１、ＣＡ３及齿状回纤维密度较之老年对照组明显下降，隔移植物使损伤侧海马大部分重获胆碱能纤维支配，联合移植物只少量提高损伤鼠ＣＡ１区纤维密度，而对ＣＡ３及齿状回无影响。隔与蓝斑联合移植的效果不如单纯隔移植。

An unilateral transection of the fimbria-fornix of aged rat was made in order to establish an animal model of senile dementia. The fetal septal or mixed septal and locus ceruleus tissue suspension was implanted to bilateral hippocampi of the lesion aged rats. After 16 weeks, ChAT and TH immunocytochemistry were used to observe the survival,growth and connection with host hippocampus of the grafts. AChE histochemistry was applied to study the distribution of the cholinergic fibers in host hippocampus and to quantify the cholinergic fiber densities in CA1,CA3 and dentate gyrus. The results showed that both septal and combined grafts could survive,the graft septal mass was larger than the combined one.Inside the graft,the abundant ChAT positive neurons distributed more in the center or along the boundary with host hippocampus.A few TH positive neurons were dispersed.The quantitative study of AChE-positive fibers showed that the mean fiber densities of CA1,CA3 and dentate gyrus were all significantly decreased in lesion ipsilateral hippocampi compared to that in control rats. The septal grafts could mostly reinnervate the lesioned hippocampi in all three areas,but the combined grafts could only increase the fiber density in CA1, it had no effect on CA3 and dentate gyrus.The combined septal with locus ceruleus graft did not act as well as the septal graft.