高效液相色谱-蒸发光散射检测法测定人参固本口服液中人参皂苷的含量

Determination of ginsenoside Rg_1 and Re in Renshenguben oral liquid by HPLC-ELSD

目的建立人参固本口服液中人参皂苷Rg1和Re含量测定的方法。方法色谱柱为C18柱;流动相为乙腈-水(22:78,调甲酸pH至2.4);流速1.0 ml.min-1;ELSD参数:漂移管温度110℃,空气流速3.0 L.min-1。结果人参皂苷Rg1在0.4～2.4μg范围内呈良好的线性关系(r=0.997 0),平均回收率为98.9%,RSD=0.32%;Re在0.2～1.2μg的浓度范围内呈良好的线性关系(r=0.998 6),平均回收率为97.7%,RSD=0.25%。结论用此法分析结果准确可靠,适用于此制剂的质量控制。

Objective To establish the method for determination of ginsenoside Rg1 and Re in renshenguben oral liquid. Method The C18 column was used .The mobile phase consisted of acetonitrile-water （22：78,adjusted to pH=2.4 with formic acid）, at a flow rate of 1.0 ml·min^-1 and the ELSD was used. Results The calibration curves were liner for ginsenoside Rg1 within 0.4 - 2.4 μg （r=0.99 0） and for ginsenoside Re within 0.2 - 1.2 μg （r=0.9986）：The average recoveries were 98.9%（RSD=0.32%） for ginsenoside Rg1 and 97.7%（RSD=0.25%） for ginsenoside Re, respectively. Conclusion The method is suitable for oral liquid. the determination of ginsenoside Rg1 and Re in renshenguben oral liquid.