从低温贮藏的玉米花粉中分离生活精细胞

The Isolation of Viable Sperm Cells from Cryopreserved Pollen of Zea mays L.

从低温（—２０℃）贮藏的玉米花粉中分离出的生活精细胞，其产量随贮藏时间的延长而降低。但是，从－２０℃贮藏２４ｈ以内的花粉，仍可分离到相当数量的生活精细胞。以新鲜花粉作对照，从贮藏８ｈ的花粉分离生活精细胞，相对产量可达６４％

Although the yield of viable sperm cell of Zea mays L.decreases with the cryopreserving time of pollen at -20℃,certain amount of the viable sperm cells could still be obtained from the frozen pollen within 24 hours.The relative sperm yield from the pollen stored at -20℃ for 8 hours could reach about 64%,compared with that from fresh pollen.