大鼠肝细胞GSH转运体系在非洲爪蟾卵中的表达

EXPRESSION OF RAT LIVER GSH TRANSPORT SYSTEM IN XENOPUS LAEVIS OOCYTES

探讨大鼠肝细胞ＧＳＨ转运体系在非洲爪蟾卵中表达的可能性。用大鼠肝细胞ｍＲＮＡ注射蛙卵，检测蛙卵转运ＧＳＨ的活性。结果：注射大鼠肝细胞ｍＲＮＡ的蛙卵，３ｄ后能表达摄取和释放ＧＳＨ的转运活性，而注射水的蛙卵则不显示这种转运活性。将大鼠肝细胞ｍＲＮＡ进一步分段离心后分别注射蛙卵，发现大鼠肝细胞的两类ＧＳＨ转运蛋白分别由分子大小为２．０～３．０ｋｂ和４．０～５．０ｋｂ的ｍＲＮＡ所编码。结论：非洲爪蟾卵可用于检测和克隆大鼠肝细胞ＧＳＨ的转运蛋白。

PURPOSE The possibility of the expression of rat liver GSH transport system in Xenopus laevis oocytes was studied.METHODS Oocytes were injected with rat liver mRNA and GSH transport activities of oocytes were measured.RESULTS The oocytes injected with rat liver mRNA required 3 days to express the activities to release or uptake GSH,while the oocytes injected with water did not show any activities.Using size-fractionation of rat liver mRNA,2 fractions were found to encode the two different GSH transporters,2.0～3.0 kilobase and 4.0～5.0 kilobase mRNAs respectively.CONCLUSIONS Xenopus Laevis oocytes may be used for studying and expression cloning of rat liver GSH transporters.