人胰腺癌组织胚胰腺泡蛋白表达的免疫组织化学研究

Immunohistologic study of feto-acinar pancreatic protein in tissues of human pancreatic cancer using monoclonal antibody J_(28)

用ＡＢＣ免疫组织化学法，以单克隆抗体Ｊ（２８）检测胰腺癌等组织中的胚胰腺泡蛋白（ＦＡＰ）。结果：正常胰腺（５例）基本无ＦＡＰ表达；慢性胰腺炎阳性率为６０．００％（６／１０），强度为；胚胎胰腺６例中全部为阳性强度；胰腺癌阳性率为７３．６８％（１４／１９），强度为；而肝胰壶腹癌（１例）、胰岛细胞瘤（１例）及胰腺转移性腺癌（５例）无ＦＡＰ表达。胰腺癌ＦＡＰ表达强度较慢性胰腺炎强（Ｐ＜０．０５）。本研究表明ＦＡＰ在胚胎胰腺中存在，正常胰腺消失，胰腺癌及慢性胰腺炎时可以再表达，其表达可能与胰腺细胞的增殖分化有关。

The immunohistologic distribution of the FAP detected by McAb J(28) using an avidin-biotin complex(ABC)technique is described. Tests were carried out on the tissues of fetal pancreas(n=6),pancreatic cancer(n=19),chronic pancreatitis(n=10),normal pancreas(n=5),metastatic tumors invading pancreas(n=5),carcinoma of ampulla(n=1) and islet cell tumor(n=1).All sections were formalin fixed and paraffin embeded.In the normal pancreas only a few positive acinar cells were found,less than 5.00%.In pancreatic cancer the expression of FAP was found in 73.68% sections.No difference was found among histological types.In pancreatitis the expressive rate of FAP was 60.00%,but the extent of staining was less than that of pancreatic cancer(P<0.05).No staining was found in metastatic tumor invading the pancreas,carcinoma of ampulla and islet cell tumor.There was a strong expression of FAP in fetal pancreas.The results show that there is reexpression of FAP in pancreatic cancer and chronic pancreatitis.