摘要
Helicoverpa armigera single nucleocapsid nucleopolyhedrovirns open reading frame 101 （ha101） is 762 nts in length and encodes a 254 amino acid peptide with predicted 29 kDa molecular weight. The homologues of ha101 were explored using BLASTP searching tool in the updated GenBank/EMBL and SWlSS-PROT databases. The results showed that the homologues of ha101 were present in all the completely sequenced lepidopteran nucleopolyhedrovirnses and granulovirnses, suggesting that ha101 might be a functional gene associated with their lepidopteran hosts. Sequence alignment of ha101 and its homologues revealed that 10 amino acids were completely conserved. RT-PCR analysis of ha101 manifested that the transcript of ha101 was first detected at 24 hpi and remained detectable at up to 122 hpi, suggesting that ha101 was transcribed during late stages of infection. Ha101 was expressed using Bac to Bac system in Tn5B-1-4 cells. The product of ha101 expressed in Tn5B-1-4 cells was approximately 29kDa, consistent with the predicted molecular weight, and the results were confirmed by western blot analysis. The subcellular localization indicated that ha101 was aggregated along nuclear envelope during the early stages of infection and spread out to the entire nucleus including virogenic stroma in late stages of infection, suggesting that ha101 may play a specific role in virion assembly process or virogenic stroma arrangement.
Helicoverpa 有佩带徽章权利的人单个 nucleocapsid nucleopolyhedrovirus (ha101 ) 开的读物框架 101 是在长度的 762 nts 并且编码 254 氨基酸肽与预言了 29 kDamolecular 重量。ha101 的相当或相同事物用在 updatedGenBank/EMBL 和 SWISS-PROT 数据库寻找工具的 BLASTP 被探索。结果证明 ha101 的相当或相同事物在所有完全定序的鳞翅类的 nucleopolyhedroviruses 和 granuloviruses 是在场的，建议 ha101 可能是与他们的鳞翅类的主人联系的功能的基因。ha101 和它的相当或相同事物的顺序排列表明 10 氨基酸完全被保存。ha101 的抄本是第一，这在 24 hpi 检测并且在多达 122 hpi 仍然保持可检测的表明的 ha101 的 RT-PCR 分析，建议那 ha101 在感染的迟了的阶段期间被抄录。哈 101 在 Tn5B-1-4 房间用用绳子拖的平底渡船被表示到用绳子拖的平底渡船系统。ha101 的产品表示了 inTn5B-1-4 房间是约 29 kDa，与预言的分子量，和结果一致被西方的污点分析证实。细胞的本地化显示了的潜水艇那 ha101was 在感染的早阶段期间聚集了原子信封并且在感染的迟了的阶段包括 virogenic 基质展开到全部原子核，建议那 ha101 可以在 virion 汇编过程或 virogenic 基质安排起一个特定的作用。
