壳寡糖对小鼠免疫系统的调节作用

Effects of oligochitosan on the immunoregulation in mice

目的研究壳寡糖对小鼠特异性体液免疫及细胞免疫功能的影响。方法壳寡糖60,120,240 mg.kg-1腹腔注射,每日1次,连续14d,检测溶血空斑数、血清溶血素水平、ConA诱导的T淋巴细胞转化率和迟发型变态反应。结果腹腔注射后2周,壳寡糖120mg.kg-1组小鼠抗体形成细胞、血清溶血素水平、淋巴细胞增殖率、脾脏体重指数及耳廓肿胀度较对照组有显著性增加。结论适当剂量的壳寡糖能提高小鼠的特异性体液免疫及细胞免疫功能。

Objective To investigate the effects of oligochitosan on the humoral and cellular immunity in mice. Methods Divided the mice into four groups, test groups were given ip oligochitosan at doses of 60,120,240 mg · kg^-1 for 14 days and the control group was given 0.90% NS. The number of haemolytie empty spots, the level of serum hemolysin, the transform rate of T lymphoeytes induced by ConA, delayed hypersensitivity were measured. Resuits Compared with the control group, indices including the number of haemolytie empty spots, the level of serum hemolysin, the transform rate of T lymphoeytes induced by ConA, spleen ratio,ears swelling degree increased obviously in the dose of 120mg · kg^-1 after two weeks of treatment. Conclusion Proper dose of and cellular immunity in mice. oligochitosan can enhance the specific humoral