摘要
应用聚丙烯酰胺凝胶等电聚焦硝酸纤维素薄膜被动蛋白转移酶免疫检测技术调查了中国4个民族5个群体(n=645)抗凝血酶Ⅲ(ATⅢ)的遗传多态性,发现在大多数中国个体中都存在ATⅢ*1等位基因,基因频率介于0.9773~0.9850,而中国群体中ATⅢ*2等位基因频率均较低,介于0.0091~0.0169。本次调查还发现了ATⅢ1种“阳极变异”和3种“阴极变异”。本研究表明中国人ATⅢ血型系统在蛋白质水平上所能提供的多态性信息量有限。
The genetic polymorphisms of antithrombin Ⅲ(ATⅢ) in 5 Chinese populations were studied by isoelectric focusing on polyacrylamide gels followed by immunoblotting. The products of two alleles at the antithrombin Ⅲ locus together with 1 ATⅢ anodic variant and 3 cathode variants were observed. The results revealed that the predominant allele of ATⅢ blood group system in Chinese populations was ATⅢ*1, with frequencies between 0.9773 0.9850. Frequencies of ATⅢ*2 ranged from 0.0091 to 0.0169. These results indicate that the polymorphic information content of ATⅢ blood group system in Chinese populations is limited.[FL)〗
出处
《华西医科大学学报》
CSCD
1996年第2期146-150,共5页
Journal of West China University of Medical Sciences
基金
国家自然科学基金资助和纽约中华医学基金
关键词
抗凝血酶Ⅲ
遗传多态性
等电聚焦
Antithrombin Ⅲ Genetic polymorphism Isoelectric focusing