大鼠系膜增殖型肾小球肾炎模型的改进

Improvement in Model of Mesangial Proliferative Glomerulonephritis of Rat

为深入探讨胃肠道粘膜免疫与系膜增殖型肾小球肾炎的关系，作者采用葡萄球菌肠毒素Ｂ（ＳＥＢ）分次静脉注射，牛血清白蛋白（ＢＳＡ）隔日灌胃及弗氏佐剂分次皮下注射，建立ＳＤ大鼠系膜增殖型肾小球肾炎模型，观察肾小球的病理变化。结果显示：自实验的第６周末大鼠肾小球出现系膜细胞及系膜基质增生，至第８周末呈现明显的系膜细胞及系膜基质增生。本模型制作时间较短（６～８周），病变稳定，模型的建立进一步证实胃肠粘膜免疫在系膜增殖型肾小球肾炎发病中起着重要的作用。

To establish a model of mesangial proliferative glomerulonephritis (MsPGN),we gave Sprague Dawley rats staphylococcal enterotoxin B(SEB) intravenously twice in different times,bovine serum albumine (BSA) through digestive tract every other day,and Freud′s adjuvant subcutaneously twice in different times,respectively.The results showed that measangial cells and matrix in glomeruli of rats began to proliferate at the end of 6th week and this change became obvious at the end of 8th week.The duration of experiment was shorter than that using only SEB,and the change of pathomorphology was stable. The experiment confirms the thesis that mucosal immunization of gastroenteric tract plays an important role in the development of MsPGN.