荧光定量PCR检测动脉粥样硬化患者血清巨细胞病毒

Real-time fluorenscent quantitative PCR detect human cytomegalovirus in atherosclerosis

目的建立荧光定量PCR(FQ-PCR)方法,用于动脉粥样硬化(AS)患者人巨细胞病毒(HCMV)感染的早期诊断.方法用FQ-PCR检测89例临床分期为2～4期的AS患者血清中HCMV(IE)基因,同时用ELISA法检测抗-HCMV抗体.结果以80拷贝/ml血清为标准,89例标本中HCMV-DNA阳性率为74.2%;抗-HCMV阳性率为39.3%,与正常对照组(50例,阳性率为12%)相比,差异有显著意义(P＜0.05).结论 FQ-PCR可作为临床AS患者早期HCMV(IE)基因检测的主要方法.

Objective To constirute the technique of real-time fluorenscent quantitative PCR for early diagnosis infection of human cyromegalovirus in azherosclerosis. Methods FQ-PCR was used to quantify HCMV-DNA of 89 AS serums defined as grade 2-4 in clinic. And ELISA was used to test anzi-HCMV. Results If 80 copies/ml was regarded as the standerd, the positive rate of 89 AS serums HCMV-DNA was 74.2 %. The positive rate of antiCMV was 39.3 %. Contrasz to normal control group（the positive rate is 12 % ）, it had significant difference（ P 〈 0. 05）.Conclusion As early and major method in clinic,the ,echnique of real-time fluorenscent quantitative PCR is found for deteczing IE gene of human cytomegalovirus in azherosclerosis.