双嘧达莫对细胞免疫的抑制作用

The effects of dipyridamole on cellular immunity

目的：观察双嘧达莫对小鼠免疫功能的影响。方法：采用小鼠末梢血白细胞计数，脾脏称重计算脾脏系数，测小鼠足垫厚度的差值确定小鼠迟发型超敏反应，用ＰＨＡ和ＣｏｎＡ诱生的脾淋巴细胞增殖测定淋巴细胞转化功能，作为活化的小鼠脾细胞测定小鼠ＩＬ－２的活性。结果：双嘧达莫１，１０，４０ｍｇ／ｋｇ抑制小鼠的迟发型超敏反应，降低小鼠末梢血白细胞计数和脾脏系数。双嘧达莫（Ｄｉｐ），１，１０，１００，１０００μｍｏｌ／Ｌ抑制ＰＨＡ诱导的小鼠脾淋巴细胞转化和ＣｏｎＡ诱导的小鼠脾淋巴细胞增殖反应，Ｄｉｐ１，１０，１００μｍｏｌ／Ｌ亦抑制小鼠脾细胞产生ＩＬ－２。结论：实验结果为Ｄｉｐ老药新用提供一定的理论依据，临床上Ｄｉｐ应用于一些免疫性疾病的治疗似有较大潜力。

OBJECTIVE:To observe the effects of dipylidamole(Dip)on murine immune function. METHlD:Murine peripheral white blood cells count , and spleen index , measured by spleen weighing were usedto detectmurine foot pad thickness difference and to define the murine delayed hypersensitivity reaction. PHA and Con A in- duced murine spleen lymphocyte mitogenesis were used to determine lymphocyte transformation function. The acti- vating murine spleen cells were used to detect the activity of IL-2. RESULTS:Dip 1, 10, 40 mg/kg ip inhibitedmurine delayed hypersensitivity reaction induced by sheep red blood cells, and obviously decreased murine spleenindex and peripheral white blood cells number. Dip 1,10, 100, 1 000 μmol/L in vitro inhibited PHA induced murinespleen lymphocyte transformation and Con A induced murine spleen T lypphocyte cells mitogenesis. Dip 1,10, 00μmol/L inhibited interleukin-2 produced by murine splenocytes ( 1×106/ml). CONCLUSION:A theoretical basis isprovided for Dip, which will have potentialities in clinical application of some immunopathy therapy.