银杏叶和山楂叶的抗氧化作用

Antioxidant action of Ginkgo biloba leaves and hawthorn leaves

目的：研究银杏叶、山楂叶提取物的抗氧化作用。方法：采用荧光法测定过氧化作用的产物丙二醛含量；分光光度法测定银杏叶、山楂叶提取物对氧自由基（Ｈ２Ｏ２）的抑制作用。结果：银杏叶（Ｇ）、山楂叶（Ｈ）的提取物能抑制小鼠肝匀浆在３７℃温育下生成的丙二醛，抑制率Ｇ为６８．５％，Ｈ为７６．９％。抑制由邻苯三酚在ｐＨ８．４自氧化产生的（与对照组相比，Ｇ：Ｐ＜０．０１、Ｈ：Ｐ＜０．０５），并能降低由Ｈ２Ｏ２所致的血红蛋白氧化和红细胞的溶血作用（溶血率Ｈ２Ｏ２为１０．９％，经Ｇ和Ｈ抗氧化作用后分别为５．７％和９．２％）。结论：银杏叶、山楂叶提取物对氧自由基具有强的抑制作用。

OBJECTIVE:To study the antioxidant action of extracts of Ginkgo biloba L.leaves(C)and hawthorm leaves(H).METHOD:The malondialdehyed(MDA)formation of peroxidant action was determined by fluorimetry.The inhibiting effect of G and H on oxygen free radical(O2,H2O2)was studied by spectrophotometry.RESULTS:The extracts of G and H could inhibit MDA formation by incubating mice liver hemogenate at 37℃(1nh%of G and H were 68.5%,76.9%respectively),and O2 produced by pyrogallic acid at pH8. 4(compare with control,G:P<0. 01,H:P<0.05). G and H could decrease hemoglobin oxidation and hemolysis by H2O2(hemolysis rate of H2O2 was 1 0.9%after the antioxidation of G and H were 5.7%,9.2%respectively).CONCLUSION:The extracts of G and H should possess an higher inhibiting action on oxygen free radicals.