血管紧张素原基因M235T多态性与老年脑梗死的相关性研究

Association of M235T Polymorphism of the Angiotensinogen Gene with Cerebral infarction in Old Patients.

目的 探讨血管紧张素原（angiotensinogen AGT）基因M235T分子变异与中国人脑梗死（cerebralinfarction CI）之间的关系。方法 采用聚合酶链反应（PCR）及限制性片段长度多态性分析（RFLP）法对75例CI、48例健康对照进行了AGT基因M235T多态性检测。结果 CI组AGT基因T235等位基因频率为0．78，235TT基因型频率为0．64，与对照组（分别为0．604和0．375）比较差异具有显著性（x^2=8.82P=0．003；x^2=8、27 P=0．004）。校正了CI的几种危险因素（血总胆固醇、血糖及年龄）后，235TT基因型仍可使CI发生的危险性增加（分别为OR=3．289，P=0．036；OR=2．49，P=0．023）。结论 AGT基因235TT型可能是中国人群CI发病的独立危险因素。

Objective To investigate the association between angiotensinogen （AGT） gene M235T polymorphism and cerebral infarction in a Chinese population. Methods AGT gene M235T polymorphism was examined in 75 cases with CI and 48 healthy controls by polymerase chain reaction （PCR） amplification and restriction fragment length polymorphism （RFLP）. Results The frequencies of AGT gene T235 allele and 235TT genetype were 0.78 and 0.64 respectively. Compared with those of the control group （0.604 and 0.375 respectively）,the difference was statistically significant （ x^2=8.82,P=0.003; x^2=8.27,P=0.004 ）. After three CI risk factors （ glycemia, cholesterol and age）had been adjusted, 235TT genotype could still raise the incidence of CI （OR=3.289, P=0.036; OR=2.49, P=0.023）. Conclusion 235TT genetype of the AGT gene might be an independent risk factor of CI in a Chinese population.