涎腺腺样囊性癌细胞分化行为的实验观察

Observationsofcelulardefferentiationbehaviourinadenoidcysticcarcimomaofhumansalivaryglandinvitro

为探讨涎腺腺样囊性癌（ａｄｅｎｏｉｄｃｙｓｔｉｃｃａｒｃｉｎｏｍａ，ＡＣＣ）中肌上皮细胞的来源和此癌的组织发生，对其细胞的一些分化行为进行了实验观察。用ＡＢＣ免疫组织化学方法，证明了ＡＣＣ－２和ＡＣＣ－３细胞系细胞，在体外能不同程度地表达肌上皮细胞特有的Ｓ－１００蛋白、肌动蛋白（ａｃｔｉｎ）和肌浆球蛋白（ｍｙｏｓｉｎ）成分；加入分化诱导剂ｄＢ－ｃＡＭＰ后，可使上述成分表达增强并使癌胚抗原（ｃａｒｃｉ－ｎｏｅｍｂｒｙｏｎｉｃａｎｔｉｇｅｎ，ＣＥＡ）由阴性转阳性。作者还观察到ＡＣＣ细胞的分化与其增殖速度呈负相关关系；改变细胞生长环境时，部分细胞呈“印戒”样，表明其具有分泌功能；将单个细胞悬浮生长于胶原凝胶为基质的培养基可形成典型的管样结构。作者认为，本研究结果支持ＡＣＣ的组织发生来自闰管细胞的观点，对认识该肿瘤的发生很有参考价值。

Theceluardiferentiationbehaviourofadenoidcysticcarcinoma(ACC)ofsalivaryglandwasinvestigatedinvitrowithimmunohistochemistry.TheresultsshowedthatthecelsofACC-2andACC-3cellinescanexpresssomemarkerproteinsinvitroincludingS-100protein,actin,myosinwhichischaracterizedasafeatureofmyoepithelialcels.Whendiferentiationinducer,dB-cAMP,wasaddedtothemonolayercelsculturedindishes,themarkerproteinexpresionincreasedandtheCEAchangedtopositive.ThecelulardiferetiationbehaviourofACCcelsisrelatedtotheproliferatespeedofcels.Somecelsmanifestedfingerring-likewhenthecultureconditionwaschanged,itmeansthatthesecelshavethesecretoryfunction.IftheACCcelsweresuspendedsinglyincolagengelswhichperformedasanextracelularmatrixtheygrewandformedthetubule-likestructure.TheresultswilhelpustounderstandthehistogenesisofACC,thesourceofmyoep-ithelialcelsinACCandthevarietyofhistologicalstructuresofACC.