蛇床子素对去卵巢大鼠近侧胫骨代谢影响的定量研究

QUANTITATIVE STUDY ON THE EFFECT OF OSTHOLE ON PROXIMAL TIBIAE IN OVARIECTOMIZED(OVX)RATS

切除３月龄ＳＤ大鼠双侧卵巢１２周后，其骨形成的参数值明显增加（％Ｌ．Ｐｍ＋５８％，ＢＦＲ／ＢＶ＋１０５％，ＢＦＲ／ＢＳ＋７４％，％０．Ｐｍ＋１８８％），同时骨吸收的参数值增加（％Ｅｒ．Ｐｍ＋１５５％），荧光标记周长与吸收周长的比率－４１％，由于骨吸收大于骨形成，骨质丢失（％Ｔｂ．Ａｒ－５９％，Ｔｂ．Ｔｈ一１４％），出现高转换型骨质疏松。分别用蛇床子素（ｏｓｔｈｏｌｅ）６．７ｍｇ·ｋｇ￣（－１）ｉｇ，每周６次，尼尔雌醇（ｎｉｌｅｓｔｒｉｏｌ）１ｍｇ·ｋｇ￣（－１）ｉｇ，每周１次，持续１２周，均能明显抑制去卵巢诱导的骨高转换，防止骨质丢失。但蛇床子素抑制骨高转换的效应比尼尔雌醇低（蛇床子素治疗组比尼尔雌醇治疗组％Ｔｂ．Ａｒ一５５％）。

Thirty-one 3-month-old ♀ Sprague -Dawley rats were randomly divided into 5groups,basal control(group 1,killed at the begining),aging control(group 2),ovariectomized(OVX,group3),OVX with nilestriol treatment group(group 4)and OVX with osthole treatmentgroup(group 5).Group 2 and group 3 ig with water 5 ml· kg -1 and group 5 ig with osthole 6.7m8· kg-1, all once a day for 6 d;group4ig with nilestriol 1 mg· kg-1,once a week.After 12weeks,all rats were killed.The proximal tibiae of rats were processed to undecalcified sections at 20um thickness for histomorphometric analysis.OVX was shown to reduce markedly the trabecular bonemass(%Tb.Ar一59%)due to increase of bone turnover with the result that bone resorption exceededbone formation, as compared with aging controls.In contrast,treatment of OVX rats with Ostholeand nilestriol increased significantly the trabecular area(increased 68%and 274%compared with thatof OVX respectively).Our results indicate that osthole and nilestriol treatment provides protectionagainst osteoporosis in OVX rats.The protective mechanism of osthole and nilestriol involvessupressiOn of bone turnover,but the effects of osthole is lower than that of nilestriol(trabecular areadecreased 55%more in osthole group than that with nilestriol treatment).Our finding may providetheoretical evidence for the clinical use of osthole or nilestriol for treatment and prevention ofosteoporosis.