超临界流体萃取法及高效液相色谱法分析延胡索中延胡索乙素的含量

ANALYSIS OF DL-TETRAHYDROPALMATINE IN CORYDALIS YANHUSUO W.T.WANG BY SUPERCRITICAL FLUID EXTRACTION AND HPLC

采用超临界流体萃取法提取延胡索及其成方制剂中的延胡索乙素，以苯作改性剂，于４０℃，４２ＭＰａ压力下，二氧化碳静态萃取５ｍｉｎ，动态萃取３ｍｌ。该法操作简便快速，提取完全，也为其它中药及其制剂中有效成分的定量分析建立了一种简便、快速、有效的提取方法。应用硅胶柱─反相洗脱系统的高效液相色谱法测定延胡索及其成方制剂中延胡索乙素的含量，方法简便、快速、精密度高。此色谱系统同样适于其它中药及其制剂中生物碱的定量分析。

A supercritical fluid extraction (SFE)method was developed for the extraction of dl-tetrahydropalmatine in Corydalis yanhusuo W.T. Wang and its patent preparations.Benzene was usedas a modifier of supercritical fluid CO2.The sample was extracted by this supercritical fluid staticallyfor 5 min and then 3 ml dynamically at 40℃ and 42 MPa. An unmodified silica gel YWG columnwith reversed phase eluent was used to determine dl-tetrahydropalmatine in Corydalis yanhusuo W.T.Wang and its patent preparations.A mixture consisting of methanol and 2 mmol·L-1 phosphatebuffer(pH 7.2)(75:25)was used as the mobile phase. The method is simple, rapid and precise, soit can be used for the extraction and determination of the alkaloids in other crude drugs andpreparatlons.