Survivin在非小细胞肺癌组织中的表达及其与临床病理特征的关系

Expression of Survivin and its Relationship with Clinicopathological Features in theNon-Small Cell Lung Carcinoma

目的探讨Survivin在非小细胞肺癌(NSCLC)组织中的表达及其与临床病理特征的关系。方法应用免疫组织化学SP方法,检测100例NSCLC组织中Survivin的表达。选取已知Survivin阳性表达的10例胃癌石蜡标本作为阳性对照,10例正常肺组织作为阴性对照,以非免疫兔血清替代Survivin作为替代对照,以PBS缓冲液替代Survivin作为空白对照。采用χ2检验分析Survivin的表达状态与患者的年龄、性别,癌组织学类型、分化程度、p-TNM分期、淋巴结转移,患者生存期的关系。结果Survivin在NSLCC组织中的阳性表达率为63.0%(63/100),不同p-TNM分期、不同生存期、淋巴结有转移和无转移的NSCLC组织中Survivin阳性表达率比较有非常显著性差异(P<0.01),患者不同年龄,不同性别,不同的癌组织学类型和分化程度,Survivin表达阳性率互相比较,均无显著性差异(P>0.05)。结论Survivin表达阳性的NSLCC患者预后不良,Survivin的表达可作为判断NSLCC患者预后的1个指标。

Objective To study the expression of Survivin and its relationship with clinicopathological features in the nonsmall cell lung carcinoma（NSCLC）. Methods The expression of Survivin were tested by immunohistochmistry in the specimens of 100 cases of NSCLC. 10 stomach cancer samples with documented Survivin（ ＋ ） and 10 samples of normal lung tissue were taken as positive and negative controls. Non-immune rabbit serum and PBS were used to replace Survivin as substitute and sham controls. The relationship between the expression of Survivln and the age,sex, histological types, differentiation, p-TNM stage, lymph nodes metastases,survival time was analysed by using the X^2 test. Results The expression rate of survivin in NSCLC was 63.0 % （63/100）. The expression of survivin was significantly associated with p-TNM stage.survival time and lymph nodal metastases（P 〈 0.01）, but not with age.sex.histologic subtype, differentiation（ P 〉0.05）. Conclusion Patients with Survivin（ ＋ ） NSCLC have a poor prognosis. The expression of survivin could be taken as one of the prognostic factors for lung cancer.