摘要
用地高辛配基(Digoxingenin)分别标记,制备HPV-6、HPV-11、HPV-16及HPV-18的DNA探针,应用核酸杂交技术,对37例宫颈癌活检组织DNA作了检测,各型HPVDNA的检出率为:HPV-60%(0/37)、HPV-112.7(1/37)、HPV-1667.5%(25/37)、HPV-1813.5%(5/37),联合应用HPV-16和HPV-18探针共同检测结果为81%(30/37)。结果显示:我国宫颈癌的发生与HPV感染密切相关,联合应用高危型HPV探针可提高检出率并可省时、简便。DIG标记探针因具特异、敏感、安全和简便诸特点,宜推广应用。
The DNAs of HPV types 6、11、16、18 were labelled with digoxingenin as molecular probes for nucleoacid hybridization , the 3 7 biopsies from patients with cervical cancer were identified with different HPV type probes, the positive rates were:HPV-6 0%(0/37), HPV-11 2.7%(1/37),HPV-16 67.5%(25/27), and HPV-18 13.5%(5/37) ,respectively, the combining use of HPV-16 and HPV-18 81%(30/37).The results showed that HPV infection was closely associated with cervical cancer. The use of high risk types probes may be more simple, rapid and positive for detection, DIGlabelled probes are specific, sensitive, safe, simple,and valuable for clinical application.
出处
《西安医科大学学报》
CSCD
1996年第1期5-7,共3页
Journal of Xi'an Medical University(Chinese)
基金
美国中华医学基金
关键词
人乳头瘤病毒
宫颈癌
地高辛配基
核酸杂交
human papillomavirus
cervical cancer
digoxigenin
nucleoacid hybridization