DIG－DNA探针检测宫颈癌组织中人乳头瘤病毒6、11、16、18型DNA

DETECTION OF HUMAN PAPILLOMAVIRUS TYPES 6、11、16、18 DNA IN BIOPSIES OF CERVICAL CANCER BY USING DIG LABELLED PROBES

用地高辛配基（Ｄｉｇｏｘｉｎｇｅｎｉｎ）分别标记，制备ＨＰＶ－６、ＨＰＶ－１１、ＨＰＶ－１６及ＨＰＶ－１８的ＤＮＡ探针，应用核酸杂交技术，对３７例宫颈癌活检组织ＤＮＡ作了检测，各型ＨＰＶＤＮＡ的检出率为：ＨＰＶ－６０％（０／３７）、ＨＰＶ－１１２．７（１／３７）、ＨＰＶ－１６６７．５％（２５／３７）、ＨＰＶ－１８１３．５％（５／３７），联合应用ＨＰＶ－１６和ＨＰＶ－１８探针共同检测结果为８１％（３０／３７）。结果显示：我国宫颈癌的发生与ＨＰＶ感染密切相关，联合应用高危型ＨＰＶ探针可提高检出率并可省时、简便。ＤＩＧ标记探针因具特异、敏感、安全和简便诸特点，宜推广应用。

The DNAs of HPV types 6、11、16、18 were labelled with digoxingenin as molecular probes for nucleoacid hybridization , the 3 7 biopsies from patients with cervical cancer were identified with different HPV type probes, the positive rates were:HPV-6 0%(0/37), HPV-11 2.7%(1/37),HPV-16 67.5%(25/27), and HPV-18 13.5%(5/37) ,respectively, the combining use of HPV-16 and HPV-18 81%(30/37).The results showed that HPV infection was closely associated with cervical cancer. The use of high risk types probes may be more simple, rapid and positive for detection, DIGlabelled probes are specific, sensitive, safe, simple,and valuable for clinical application.