喀斯特高海拔山区玉米骨干自交系遗传多样性RAPD标记分析

Analysis on Genetic Diversity of Maize Backbone Inbred Lines in Karst High Elevation Mountainous Area by RAPD Markers

以代表我国玉米6个主要杂种优势群旅大红骨、Lancaster、Reid、苏湾、墨白和塘四平头的标准测验种丹340、自330、792 2、苏37、4 4 9、黄早四和适应我国喀斯特高海拔山区的玉米骨干自交系为材料,利用RAPD标记对其进行遗传多样性和杂种优势群划分。从90个随机引物中筛选出2 1个多态性好的引物扩增材料,共产生14 6条谱带,其中12 4条谱带有多态性,占86 3% ,说明喀斯特高海拔山区的玉米骨干自交系具有较丰富的遗传多样性。通过UPGMA聚类分析,以遗传相似系数为0 6 32 ,可将我国喀斯特高海拔山区玉米种质资源的16个骨干自交系划分为5个类群。

Twenty inbreds were classified by RAPD markers（random amplified polymorphic DNA procedures）. The materials included six main inbreds （Dan 340, Zi 330, 7922, Su37, 449 and Huangzaosi）, which represented six main heterotic groups（Luda Red Cob, Lancaster, Reid, Suwan, Mobai and Sipingtou）of maize backbone inbred lines in karst high elevation mountainous area in China. Total 136 DNA polymorphic bands were produced from 21 primers which were screened out from 90 random primers, and an average proportion of polymorphic loci was 86.3 %. Cluster analysis by unweighted pair group method of arithmetic means（UPGMA） suggested that 20 inbreds could be divided into 5 groups with genetic distance from 0. 632.