高效液相色谱法测定茵虎清肝颗粒中大黄素、大黄酚的含量

Determination of Emodin and Chrysophanol in Yinhu Qinggan Granules by HPLC

目的:建立测定茵虎清肝颗粒中大黄素、大黄酚含量的方法.方法:采用高效液相色谱法,以Inertsil ODS-3 C18柱为固定相,甲醇-0.1%磷酸溶液(90:10)为流动相,检测波长254 nm,流速1.0 ml/min,柱温室温.结果:大黄素在69.76～348.80 ng(r=0.999 6),大黄酚在12.08～60.40 ng(r=0.999 9)范围内呈良好线性关系,平均回收率大黄素为98.97%,RSD为1.16%;大黄酚99.72%,RDS为2.06%.结论:该方法简便,准确,重现性好,可用于测定茵虎清肝颗粒中大黄素、大黄酚的含量.

Objective：To establish a method for determination of the contents of emodin and chrysophanol in Yinhu Qinggan Granules. Methods： HPLC medthod was selected to determine the contents of emodin and chrysophanol. The chromatographic conditions included lnertsil ODS-3 C18 column, methanol-（0.1% ） phosphoric acid solution （90：10） as the mobile phase, wavelength of detector at 254 nm, flow rate 1.0 ml/min and column temperature at room temperature. Results：The calibration curves were linear over the range of 69.76-348.80 ng（ r = 0. 9996）for emodin and 12.08-60.40 ng（r=0.9999） for chrysophanol ;and the average recoveries were 98.97% （ RSD = 1.16% ） and 99.72% （ RSD = 2.06% ）. Conclusion： This method is simple, accurate and reliable,which could be used for determination of the contents of emodin and chrysophanol in Yinhu Qinggan granules.