摘要
目的:建立测定茵虎清肝颗粒中大黄素、大黄酚含量的方法.方法:采用高效液相色谱法,以Inertsil ODS-3 C18柱为固定相,甲醇-0.1%磷酸溶液(90:10)为流动相,检测波长254 nm,流速1.0 ml/min,柱温室温.结果:大黄素在69.76~348.80 ng(r=0.999 6),大黄酚在12.08~60.40 ng(r=0.999 9)范围内呈良好线性关系,平均回收率大黄素为98.97%,RSD为1.16%;大黄酚99.72%,RDS为2.06%.结论:该方法简便,准确,重现性好,可用于测定茵虎清肝颗粒中大黄素、大黄酚的含量.
Objective:To establish a method for determination of the contents of emodin and chrysophanol in Yinhu Qinggan Granules. Methods: HPLC medthod was selected to determine the contents of emodin and chrysophanol. The chromatographic conditions included lnertsil ODS-3 C18 column, methanol-(0.1% ) phosphoric acid solution (90:10) as the mobile phase, wavelength of detector at 254 nm, flow rate 1.0 ml/min and column temperature at room temperature. Results:The calibration curves were linear over the range of 69.76-348.80 ng( r = 0. 9996)for emodin and 12.08-60.40 ng(r=0.9999) for chrysophanol ;and the average recoveries were 98.97% ( RSD = 1.16% ) and 99.72% ( RSD = 2.06% ). Conclusion: This method is simple, accurate and reliable,which could be used for determination of the contents of emodin and chrysophanol in Yinhu Qinggan granules.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2005年第12期1249-1250,共2页
Lishizhen Medicine and Materia Medica Research