乌龙丹流浸膏质量标准研究

Quality Standard of Wulongdan Liquid Extract

目的:建立乌龙丹的质量控制方法.方法:应用TLC法对首乌、黄芪进行定性鉴别,应用高效液相色谱法测定葛根素的含量.色谱条件:流速1 ml/min,流动相:甲醇∶乙腈∶0.025 mol/L磷酸20∶4∶76,检测波长:250 nm.结果:薄层色谱定性方法简便、灵敏、专属性强;葛根素在0.052 5～0.735μg范围之内呈良好的线性关系,r=0.999 5.平均回收率99.18%,RSD=1.55%.结论:此法简便、准确,可有效控制产品质量.

Objective ：To build up the quality control method for Wulongdan Liquid Extract. Methods：Qualitative determination of Radix Pueriae and Radix Polygoni Multiflori. Puerarin was carried out by HPLC. The mobile phase was ethanol-acetonitrile 0.025 mol/L phosphoric acid （20： 4： 76） at a flow of 1.0ml/min, and detection wavelength was 250nm. Results： TLC qualitative methods were simple, sensitive and specific, the determination method for puerarin had a good linear relationship in the range of 0.0525-0.735μg, r=0.9995. The average recovery was 99.18% ,RSD=1.55%. Condusion： This method is simple, accurate and can be used as the standards for quality control of this product.