双通道RP-HPLC法测定黄金搭档组合维生素片中VB_1、VB_2和VB_6的含量

Determination of VB_1,VB_2,VB_6 in golden partner vitamins tablet by double channel RP-HPLC

目的建立黄金搭档组合维生素片中维生素B_1、B_2和B_6含量测定方法。方法用双通道反相高效液相色谱法进行分析。色谱柱为Lichrospher5-C18柱ODS(5μm,250mm×4.6mm),流动相为水-甲醇-0.25mol·L^(-1)庚烷磺酸钠溶液-乙酸-7%氨水(70∶30∶1.5∶1∶0.25),稀释液:同流动相,检测波长VB_1248nm、VB_2270nm、VB_6280nm。结果峰面积与含量有良好的线性(γ_(VB1)=0.999920,γ_(VB2)=0.999993,γ_(VB6)=0.999998);重复性好(批内RSD_(VB1)=0.89%,RSD_(VB2)=0.42%,RSD_(VB6)=0.37%;批间RSD_(VB1)=0.83%,RSD_(VB2)=0.36%,RSD_(VB6)=0.41%);回收率高(X_(VB1)99.3%,RSD=0.91%;X_(VB2)100.1%,RSD=0.25%;X_(VB6)99.6%,RSD=0.46%)。结论方法稳定,简便易行,快速准确,可作为此片剂质量检测方法。

Aim To establish a double channel RP-HPLC method for determination of VB1 , VB2, VB6 in Golden Partner. Methods The chromatography condition was ： lichrospherS-C 18-ODS （ 5um, 250×4.6）, water-methanol-0.25 mol·L^-1 sodium 1 -heptanesulfonate -acetic acid-7% ammonium hydroxide （70： 30： 1.5：1：0.25 ）. Detection wave length was VB1 248 nm, VB2270 nm,VB6280 nm. Results The linear regression is good between area and contents （γ VB1= 0. 999920, γ VB2 = 0. 999 993, γ VB6 = 0.999 998 ） ,The reproduction is good （intra-batch RSD VB1 = 0.89% RSDvm = 0.42%, RSD VB6= 0.37% ; inter-batch RSD VB1= 0.83%, RSD VB2= 0.36% RSD VB6 = 0. 41% ）, The recovery is good （ X VB1 99.3 %, RSD = 0.91% ,X VB2 100.1%, RSD=0.25 %, X VB6 99. 6%, RSD = 0.46% ）. Conclusion The method is simple, rapid and accurate. It is applied to determinate contents of VB1 , VB2, VB6 in Golden Partner.