大鼠局灶性脑缺血/再灌注后NF-kB表达和细胞凋亡

NF-kB expression of the and apoptosis after cerebral ischemia/reperfusion

目的:研究脑缺血再灌注后大鼠脑组织半暗区核因子(nuclear factor kappa B,NF-kB)基因表达的变化与神经细胞凋亡的关系。方法:用插线法制作大鼠大脑中动脉(M CAO)栓塞/再灌注模型,原位杂交法检测大鼠脑重度缺血(3h)及再灌注损伤不同时间点(2、3d)半暗区NF-kB表达;TUNEL法测定凋亡细胞。结果:脑缺血再灌注后,缺血半暗区NF-kB的表达明显升高(P<0.01)。凋亡细胞数量亦显著增加(P<0.01)。细胞凋亡的时程变化与NF-kB的表达基本一致。结论:局灶性重度脑缺血再灌注后可引起NF-K b表达的增加,参与缺血再灌注神经细胞损伤机制。

Objective： To study the relation of the expression of NF-kB protein and the number of apoptotic cell after cerebral ischemia/reperfusion. Methods： The animal model of middle cerebral artery （MCA）ischemia/reperfusion was used. We observed that alteration of apoptosis （TUNEL method）,the expression of NF-kB （in situ hybridization method） and percent containing water （Hallenbeck method）, percent infarct volume （TTC method ）. Results： The number of NF-kB increased obviously after 3h ischemia and 24h/48h reperfusion,and at the same time the number of apoptotic cell increases obviously. Conclusion： The increase in the number of apoptotic cell is correlated with the increase in the number Of the express of NF-kB.