大鼠海马快速点燃模型的建立及机制研究

The establish and mechanism study of rapidly recurring hippocampal kindling in rats

目的：建立大鼠海马快速点燃模型并对其机制进行初步探讨。方法：制备大鼠海马快速点燃模型；观察经典抗杏仁核点燃药物对该模型的影响；观察大鼠海马快速点燃模型和大鼠杏仁核点燃模型海马CA_3区和皮层区c-fos蛋白的表达情况及蛋白抑制剂对其表达的影响。结果：大鼠海马快速点燃模型点燃成功率与刺激参数有关；苯巴比妥20，50mg·kg^1 及地西泮2，5mg·kg^(-1)对大鼠海马快速点燃ADD和Racine＇s分级有抑制作用(P＜0．05)；与正常组比较，海马快速点燃大鼠和杏仁核点燃大鼠海马CA_3区、大脑皮层c-fos免疫阳性细胞增多(P＜0．05)，在蛋白抑制剂作用下，海马c-fos免疫阳性细胞减少，但仍比正常组高(P＜0．05)。结论：大鼠海马快速点燃成功率与刺激参数具有相关性；传统抗点燃药物具有抗海马快速点燃作用；大鼠海马快速点燃模型c-fos蛋白在不同脑区和核团的免疫阳性表达细胞增加。

Objective： To establish rapidly recurring hippocampal kindlingmodels in rats. To investigate the mechanism of the models primarily. Methods：Rapidly recurring hippocampal kindling models were established in differentparameter. The effect of general anti-kindled drugs such as diazepam andphenobarbital were observed on rapidly recurring hippocampal kindling models inrats. The number of c-los immune-positive cells in CA3 of hippocampus andspecific cerebral cortex immunohistochemistrily in rapidly recurring hippocampalkindling and amygdala kindling models were calculated. Applying CHX 2h before thekindling stimulus, calculate the number of c-los immune-positive cells in the samezone. Resuits： Rapidly recurring hippocampal kindling models were establishedsuccessfully. We found that the kindled ratio was decided by stimulus parameters. Phenobarbital at doses of 20, 50mg · kg^- 1 ip and diazepam at doses of 2, 5mg · kg^-1 ip, can decrease the Racine＇s stages in rapidly recurring hippocampal kindled rats （P〈0.05）. The number of c-los immunepositive cells in CA3 of hippocampus and specific cerebral cortex increased in rapidly recurring hippocampal kindling and amygdala kindling rats （P〈0.05）. The number of c-los immune-positive cells in the same zone in rapidly recurring hippocampal kindled rats applied CHX decreased （P〈0.05）. Conclusion： Rapidly recurring hippocampal kindling models were established successfully. We found that the kindled ratio was decided by stimulus parameters. The number of c-los immune-positive cells in different zone of rapidly recurring hippocampal kindling model increased.