稀土离子镧与镨对人舌癌细胞的抑制作用

Inhibition of Lanthanum and Praseodymium Ions on Human Tongue Carcinoma Tca8113 Cell Line

目的:观察稀土离子镧与镨(LaCl3、PrCl3)对体外培养的人舌癌Tca8113细胞的影响及两种稀土离子作用的差别。方法:!用三种浓度的LaCl3、PrCl3处理体外培养的人舌癌Tca8113细胞并测定细胞生长曲线、用MTT法检测细胞增殖活性、以琼脂糖凝胶电泳和流式细胞仪检测细胞凋亡。结果:!Tca8113细胞经镧与镨离子作用后细胞增殖活性明显下降并出现明显的细胞凋亡;镧与镨对细胞的抑制作用比较差异无显著性。结论:稀土离子镧与镨对人舌癌Tca8113细胞有生长抑制作用,且作用呈剂量依赖性和时间依赖性;二者均能引起细胞凋亡,细胞凋亡率也呈剂量依赖性。两种稀土离子的细胞抑制作用差异无显著性。

Objective： To observe the influences of LaCl3 and PrCl3 on human tongue carcinoma Tca8113 cell lines in vitro and the difference between the inhibitive effects of these two rare-earth ions. Methods： Tca8113 cells cultured in vitro were treated with the different concentrations of LaCl3 and PrCl3, and the growth curves of Tca8113 cells were obtained. The proliferation rates of Tca8113 cells were detected with MTF assay. Apoptosis of Tca8113 cells treated by LaCl3 and PrCl3 was ascertained by flow cytometry and nucleus DNA electrophoresis. Results： The proliferation of Tca8113 cells treated by various concentration of LaCl3 and PrCl3 was obviously suppressed, and some cells presented apoptosis. There was no significant difference between the cell inhibitive ability of these two rare-earth ions. Conclusion： The growth inhibition and apoptosis of Tca8113 cell can be achieved through the pre-treatment of LaCl3 and PrCl3, but the effects depend on these two rare-earth ions＇ concentrations and treatment time.