ACE基因多态性与T2DM患者股动脉内中膜厚度的关系

Association of Angiotensin I-converting enzyme Gene Polymorphisms with the Carotid Intima-media Thickness in Type 2 Diabetes Mellitus

目的探讨人类血管紧张素转换酶(ACE)基因插入/缺失多态性(I/D)与2型糖尿病(T2DM)患者股动脉内中膜厚度(FA-IMT)的关系。方法采用限制性片段长度多形态多聚酶链式反应(PCR-RFLP)技术检测了303例T2DM患者和93例健康个体ACE基因内含子16插入/缺失(I/D)多态性,利用B型超声检测T2DM患者FA-IMT的情况。结果与健康对照组比较,T2DM患者I等位基因频率显著增高,而D等位基因频率显著降低。携带ACE DD基因型者FA-IMT增厚的比例显著高于携带II及ID基因型者(P<0.01);多元线性逐步回归分析显示,与T2DM患者基线FA-IMT关系密切的指标为年龄、ACE DD基因型、饮酒史。结论ACE DD基因型是T2DM患者FA-IMT增加的独立的危险因素。

Objective To investigate the relationship between angiotensin Ⅰ-converting enzyme（ACE） inserting/defaulting （I/D） gene polymorphisms and the femoral artery intima -media thickness （FA-IMT） in patients with type 2 diabetes mellitus （T2DM）. Methods The polymorphisms of ACE （I/D） was determined by polymerase chain reaction-restriction fragment length polymorphism （ PCR - RFLP） method and the FA-IMT was assessed using non - invasive high resolution B-mode uhrasonography in 303 patients with T2DM in Hunan province. Results The frequency of I allele of ACE gene polymorphisms was higher in T2DM than that in healthy controls, but frequency of D allele was lower in T2DM than that in healthy controls（P〈0.05）. Patients with ACE DD genotype had higher FA-IMT than those with ACE II or ACE ID genotype（P〈0.01）. Multivariate linear regression analysis showed that age, alcohol intake and ACE DD genotype were closely correlated with the FA-IMT in T2DM patients. Conclusion The ACE I/D gene polymorphism was a inhered risk factor for T2DM development and ACE DD genotype was one of the independent risk factors for FA-IMT in T2DM patients.