应用组织微阵列技术研究细胞周期调控因子p27^kip1和p16^Ink4a在宫颈腺癌中的表达

Expression of the cell cycle regulator p27^kip1,p16^Ink4a in Cervical Adenocarcinoma using tissue microarray

目的 研究细胞周期调控因子p27^kip1、p16^Ink4a在宫颈腺中的表达及临床意义。方法 应用组织微阵列技术结合免疫组化（二步法）检测106例宫颈腺癌组织和22例慢性宫颈炎组织中p27^kip1和p16^Ink4a的表达。结果 宫颈腺癌组织中p27^kip1蛋白阳性表达率为58．5％，明显低于慢性宫颈炎组织（P〈0．05），p16^Ink4a在宫颈腺癌组织中的阳性表达率为70．8％，明显高于慢性宫颈炎组织（P〈0．01）；p16^Ink4a蛋白表达与宫颈腺癌病理分级和组织学类型无明显相关性；p27^kip1蛋白表达与官颈腺癌病理分级无明显相关性，而与组织学类型相关，透明细胞腺癌p27^kip1阳性表达率明显低于其它类型宫颈腺癌（P〈0．05）。结论 p27^kip1和p16^Ink4a在宫颈腺癌的癌变发生过程中起重要作用，二者可能作为宫颈腺癌恶性化的参考指标。

Objective To study the expression of the cell cycle regulator p27^kip1, p16^Ink4a in cervical adenocarcinoma. Methods The expressions of p27^kip1 and p16^Ink4a were determined by tissue microarray combined with immunohistochemistry in 106 cases of cervical adenocarcinoma and 22 cases of cervical chronic inflammation. Results The positive rates of p27^kip1 protein in cervical adenocarcinoma were 58.5%,which was significantly lower than that in cervical chronic inflammation （P〈0.05） . The positive rates of p16^Ink4a protein in cervical adenocarcinoma were 70.8%,which was significantly higher than that in cervical chronic inflammation （P〈0.01）. The expression of p16^Ink4a wasn＇t related to the histological grade and type. The expression of p27^kip1 wasn＇t also related to the histological type, but the level was related to the histological type, the expression of p27^kip1 was significantly higher in clear-cell adenocarcinoma than in endocervix adenocarcinoma and endometrioid adenocarcinoma （P〈0.05）. Conclusions The expression of p27^kip1 and p16^Ink4a may play an important role in the occur of cervical adenocarcinoma, which might be the reference markers for the transformation of cervical adenocarcinoma from benign to malignant status.