摘要
在棉花组织培养中,时常有畸形胚的发生。根据畸形胚的表型,可分为九类:联体胚、子叶畸形胚、胚轴畸形胚、单极胚、多极胚、无极胚、重新愈伤化胚、玻璃化胚和白化胚。畸形胚的发生频率很高,可达94.0％。培养时间和培养基组成等均影响畸形胚的发生。通过调整培养基中激素含量可有效地使畸形胚萌发转化为正常苗。
Abnormal embryos usually appear during the process of cotton tissue culture. According to appearance, their could be classified into nine kinds: linked-embryo, cotyledon-abnormal embryo, hypocotyle-abnormal embryo, one-growth-point embryo, multi-growth-point embryo, no-growth-point embryo, vitreous embryo, callus embryo and albino embryo. The occurrence frequeney of abnormal embryos was vary high, the highest could be 94. 0%. Culture time, medium composition could effect the occurrence of abnormal embryos, and they could be transform into normal seedlings by regulating the kinds and concentrations of phytohormones in the medium.
出处
《作物学报》
CAS
CSCD
北大核心
1996年第1期107-111,共5页
Acta Agronomica Sinica
关键词
棉花
组织培养
畸形胚
转化
Cotton
Tissue culture
Abnormal embryo
