人钠碘转运体mRNA在良恶性甲状腺疾病中表达的研究

Expression of human sodium iodide symporter mRNA in benign and malignant thyroid disease

目的探讨人钠碘转运体(hNIS)mRNA在良恶性甲状腺疾病中的表达及其在疾病诊断中的价值。方法24例G raves病,27例桥本氏甲状腺炎,21例亚急性甲状腺炎,24例无功能性甲状腺结节性增生,22例无功能性腺瘤,21例乳头状甲状腺癌及17例滤泡状甲状腺癌(其中4例颈部淋巴转移),经细针抽吸甲状腺组织,用实时荧光定量PCR法测定hNIS mRNA的表达。结果良性与恶性甲状腺疾病间hNIS mRNA表达量无显著差异(P>0.05),各组间亦无显著差异(P>0.05)。4例颈部淋巴转移病灶其原发癌表达阳性,但只有2例转移灶表达阳性。结论hNISmRNA定量检测不能用于良恶性甲状腺疾病的鉴别。颈部转移淋巴中有部分未表达hNIS mRNA而原发灶有表达,其机理值得探讨。

Objective To explore the expression of human sodium iodide symporter （hNIS） mRNA in benign and malignant thyroid disease and the value of diagnosing disease. Methods. Used fine needle aspiration to obtain thyroid tissues from 24 patients with Craves disease ,27 patients with Hashimoto＇ s thyroiditis ,21 patients with subacute thyroidifis ,24 patients with nonfunctioning thyroid nodulous hyperplasia ,22 patients with nonfuncfioning adenoma ,21 patients with papillary thyroid cancer and 17 patients with follicle cancer （ concluding 4 patients with cervix lymph metastasis）. Detected the expression of hNIS mRNA using the method of real-time RT-PCR fluorescence quantitative analysis. Results The quantity of hNIS mRNA in benign and malignant thyroid disease was not significant difference（ P 〉0.05） ,also in respective group（ P 〉 0.05 ）. The expressions of two metastatic patients were positive although the primary cancers of four cervix lymph metastatic patients were positive. Conclusion The quantity of hNIS mRNA was not used in identification between benign and malignant thyroid disease, hNIS mRNA was not expressed in parts of metastatic lymphs while expressed in primary patients and the mechanism was worth exploring.