摘要
用传染性支气管炎病毒(IBV)H120、H52、M41、ARK、澳大利亚T株、野外分离株RS、RY及鸡新城疫病毒(NDV)、鸡传染性法氏囊病毒(IBDV),鸡传染性喉气管炎病毒(ILTV)、减蛋综合症病毒(EDSV)等分别接种9日龄SPF鸡胚,37℃孵育48小时后,将其尿囊液离心,沉淀用PBS悬浮,涂片,用抗IBV单抗MC作一抗、进行间接荧光抗体检测。结果:H12O、H52、M41“ARK、T株及RS、RY均显阳性,而NDV、IBDV、ILTV、EDSV均为阴性。结果表明、用此法检测鸡胚尿囊液中IBV.具有快速、敏感、特异的优点。
Abstract An indirect immunofluorescent (llF)assay based on monoclonal antibody for the rapid fdetection of infectious Bronchitis Virus (IBV)in allantoic cells was developed,Each sample,including 5 reference strains: H120, H52, M41, Ark., Aust-t and 2 yield isolates: RS, RY julia.innoculated into the allantoic cavity of about 6 nine-day-old specific pathQgen--frP.e(SPyl).'eggs,The allantoic fluids; were collected af.ter 48 h at 37C and centrifuged at 700g and 4℃for 10 min,Pelleted cells fwere rewuspended in phosphate buffered saline (PBS) placed as,drops on glass slides,The IIF was carried out by adding MAb Me against IBV aS the first antiboy, The results shown that H12.0, IH52, M41, ArK, Aust T, RY, RS are all positive, and coincided with HA,egg passage and/or experimental infection of chicken,T.he specificity of thipIIF assay had been well c.orroborated by the cross rg.action test wit-h5Pq,gilgiens containingNewcastle disease virus (NDV), Infectious laryngotracheitis vurys (\ILTVf)i Egg drop st4';:drome-76 (EDS-76) virus and infectious Bursal Disease (IBDV),teSpteetiv6ly\, TIle m.th64: \provides a simple,reliable, sensitive and inexpensive procedure for the id..tifice-tioln e,nd' de;'tection of IBV in samples.