MMP-2和MMP-9在急性白血病骨髓细胞中的表达及与白血病髓外浸润的相关性

Relationship between the expression of MMP-2 and MMP-9 in acute leukemia cells and leukemia infiltration

目的探讨基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)与急性白血病发生和髓外浸润的相关性。方法采用生物素化鼠抗地高辛标记的原位杂交法检测MMP-2和MMP-9在初治、复发、完全缓解的急性白血病患者和正常对照者的骨髓原代细胞中的表达。结果MMP-2和MMP-9在初治,特别是有髓外浸润表现者和复发急性白血病骨髓原代细胞中的表达显著高于完全缓解的急性白血病和正常对照者;关于亚型的研究发现,初治急性淋巴细胞白血病组和急性非淋巴细胞白血病(M4+M5)组中的表达显著高于急非淋(非M4+M5)组。结论MMP-2和MMP-9可能通过增强白血病细胞降解细胞外基质(ECM)等机制参与急性白血病的发生和髓外浸润过程,并在其中发挥重要作用。

Objective：To investigate the expression of MMP-2 and MMP-9 in acute leukemia and normal control and explore the relationship among MMP-2,MMP-9 and leukemia pathogenesis and infiltration. Method： The expression of MMP-2 and MMP-9 in bone marrow cells of untreated acute leukemia, relapse stage of acute leukemia, complete remission （CR） stage of acute leukemia and normal control were examined by in situ hybridization. Result：The positive rate of MMP-2 and MMP-9 in untreated, especially with infiltration and relapse stage of acute leukemia are significantly higher than that in CR stage of acute leukemia and normal control, the positive rate of MMP-2 and MMP-9 in untreated acute lymphoblastic leukemia（ALL） and acute nonlymphoblastic leukemia （ANLL） （M4 ＋M5 ）group are significantly higher than that in ANLL （non M4 ＋ M5 ）group. Conclusion： MMP-2 and MMP-9 may play an important role in leukemia pathogenesis and infiltration by enhancing the degradation of ECM.