CEIA-LIF法在重型再生障碍性贫血患者干扰素-γ水平检测中的应用

The application of CEIA-LIF in the detection of IFN-γ level in patients with SAA

目的检测再生障碍性贫血(再障)患者单个CD8+T细胞内干扰素-γ(IFN-γ)的绝对含量。方法用毛细管电泳免疫分析激光诱导荧光法对再障患者外周血单个CD8+T细胞内IFN-γ的水平进行定量检测。结果2例重型再障(SAA)患者免疫治疗前外周血单个CD8+T细胞内IFN-γ的水平分别为(151.53±28.92)z mol和(223.72±45.23)z mol,免疫治疗后相应的降为(45.57±13.90)z mol和(36.44±9.66)z mol,正常对照为(47.47±17.97)z mol。经统计分析,SAA患者治疗前、后及与正常对照比较差异均有统计学意义。结论毛细管电泳免疫分析激光诱导荧光法可以检测到外周血单个CD8+T细胞内IFN-γ的z mol水平,有望成为一种新的检测方法应用于临床。

Objective：to detect the absolute IFN-γ content in single CD8^＋ T cell of patients with SAA. Method： We used CEIA-LIF to detect the IFN-γ level in single CD8^＋ T cell from peripheral blood in patients with SAA. Resuit：The IFN-γ levels in single CD8^＋ T cell of 2 patients with SAA before immunosuppression treatment were （151.53±28.92） zmoland （223.72±45.23） z mol, respectively, then reduced to （45.57±13.90） z mol and （36.44±9.66） z mol after treatment. As a control, the normal was 27.47±17.97 z mol. There existed significant difference between the above data. Conclusion： CEIA-LIF can detect the IFN-γ level in single CDS＋T cell from peripheral blood in minimum level of zmol, and be expected to be a new method used in clinic.