TLC－荧光分光光度法测定尿中氧氟沙星的排出速率

Determination of Excretion Rate of Ofloxacin in Urine by TLC-Fluorospectrophotometry

本文报道了用ＴＬＣ－荧光分光光度法测定２名健康受试者尿中氧氟沙星的排出速率。薄层板为硅胶Ｇ板，展开剂为苯－甲醇－甲酸（１．６７∶５∶３．３３）。尿样经薄层层析后，收集氧氟沙星的斑点，用乙酸乙醇－水（９∶３∶１０）洗脱，于λｅｘ＝２９４ｎｍ、λｅｍ＝４９５ｎｍ处测定其荧光强度，在２０～８００μｇ／ｍｌ浓度范围内呈线性，回归方程为Ｆ＝０．１３＋０．０５５Ｃ（ｒ＝０．９９９３），最低检出限为１０μｇ／ｍｌ。该法灵敏、重现性好，生物样本易获取。

The excretion rate of ofloxacin in 2 healthy female volunteer urine was assayed by TLC-fluorospectrophotometry. The sample was separated on silica gel G plate using benzene-methanol-formic acid (1.67∶5∶3.33) as developer. The spot of ofloxacin was collected and extracted with acetic acid-ethyl alcohol-water (9∶3∶10), and then assayed at λex=294nm, λem =495nm. The ofloxacin concentration is linear over the range of 20～800μg/ml. The detection limit is 10μg/ml. This method is sensitive and reproducible.