快速盐析法提取DNA在HLA基因分型中的应用

A rapid salting out precedure for extracting DNA used in HLA genotyping

采用快速盐析方法提取人类有核细胞DNA，并在临床肾移植的HLA基因配型中应用。通过对210份不同组织来源的样本与标准酚氯仿法同步对比研究显示:快速盐析法提取模板DNA，无污染和有害作用；操作简单、快速，总耗时110分钟；方法稳定可靠，无一例失败；所获DNA质量好，纯度高，达到酚氯仿法所获DNA的水平；用于临床肾移植基因配型均获成功，与酚氯仿法结果完全相同。证实该法提取的DNA适用于临床器官移植基因水平的配型。

This article reported a rapid salting out procedure for extracting DNA from human nucleated cells and clinical practice in donor-recipient matching by HLA genotyping. This method was compared to the standard phenol/chloroform extraction in 210 samples from different tissues.The results showed that the salting out method was a simple, rapid and nontoxic extraction procedure. The methodwas per formed in an overall time of 110 minutes. The DNA quality obtained by this simple technique was comparable to that obtained by phenol-chloroform extraction. None of the 210 tests were failed. The DNAs obtained by both methods have been successfully used for donor-recipient genotyplng. The two methods showed completely concordant results. It was demonstrated that the DNA extracted by the rapid salting out method was suitable for clinical DNA typing in tissue and organ transplantations.