DNA碱变性增色效应及其老化性改变的实验研究

Experimental study of hyperchromic effect of alkali denaturation DNA and its ageing changes

采用变性法测定青、老龄小鼠和青、老龄大鼠各组肝ＤＮＡ的增色效应。结果表明：老龄小鼠肝ＤＮＡ碱变性增色效应高于青龄小鼠，老龄大鼠肝ＤＮＡ碱变性增色效应高于青龄大鼠，差异均有显著性意义（Ｐ＜０．０１）．这提示动物衰老时ＤＮＡ碱变性增色效应增高。

Hyperchromic effect of DNA in the liver of young and old rats or mice were determined by the alkali denaturation merhod. The results showed that the hyperchromic effect of alkali denaturation DNA in the liver of old rats or mice is obviously higher than that of the young rats of mice(P<0. 01). It is believed that the hyperchromic effect of DNA in liver increases with age.