幽门螺杆菌对胎儿胃粘膜上皮细胞的粘附用研究

Studies on adherence of H pylori to human embryonic stomach membrane

实验选用６至７个月龄的脑儿胃粘膜组织进行有关幽门螺杆菌（Ｈｐ）的粘附部位及菌株间粘附能力差异的研究，发现ＣＡＰＭＤ３２株与ＮＣＴＣ１１６３７株的粘附部位相似，Ｈｐ对胃窦及胃体下部粘膜组织具有很强的粘附能力，而对胃体上部及胃底部粘膜组织的粘附能力很差或不能粘附；而ＣＡＰＭ Ｚ－４株则对胃体、胃窦及胃底部粘膜上皮组织均表现出很强的粘附能力；表明Ｈｐ的粘附存在明显的部位特异性，不同Ｈｐ菌株间在粘附同一胎儿胃粘膜组织时表现出明显的差异，Ｈｐ与胃粘膜上皮细胞间的粘附过程比较复杂，至少包括两种类型，参与Ｈｐ粘附的粘附素和相应受体不止一种。结果提示Ｈｐ相关性慢性胃炎的好发部位及严重程度除与人胃粘膜组织不同部位细菌粘附受体的表达存在明显的差异外，还决定于Ｈｐ的粘附素的种类和粘附特性。不但首次在机体和细菌两个方面揭示了Ｈｐ相关性慢性胃炎的发病规律，也为进一步开展该菌疫苗的研制、开展对Ｈｐ感染及其相关疾病的防治提供了重要线索。

The therapeutic advances in Helicobacter pylori infection is hampered due to the lack of suitable animal model systems. The aim of this study was to further examine the binding of H. pylori to human gastric epithelial cells , we developed an in vitro bacterial adherence assay with human embryonic stomach mucosa. The mucosal sltecimens were taken from aborted human fetus ( 6～ 7 months old ) . Pieces of tissue were infected with H .pylori in vitro , some pieces of infected tissue were observed with scanning electronmicroscopy ,and some were put into culture immediately. Total number of H .pylori firmly attached were checked during the cultivation period of 72 hours We found that NCTC 11637 and CAPM D32 strains only attached to the lower body and pyloric stomach epitheliocytes ,but the CAPM Z-4 strain could attach to every parts of gastric mucosa. We would favor the hypothesis that there would be probably differ-ent 'adhesins' within different H. pylori strains , and the corresponding receptors might distribute to different parts of gastric mucosa. Further studies are needed to confirm these hypotheses , and much knowledge needs to be gained about 'adhesins' of H pylori before development of effective therapy and vaccine.