摘要
在细胞单层培养系统的基础上,将瘢痕组织来源的成纤维细胞培养在胶原凝胶中,形成了瘢痕成纤维细胞的三维培养系统。这一系统使细胞、细胞外基质以及各种调控因素共同构成了一个有机整体,成纤维细胞在这一整体中所表达的生物学行为更加接近于它们在活体瘢痕组织中的行为。运用成纤维细胞三维培养系统不仅可以观察和研究细胞的形态、生长增殖和物质代谢,还可以研究细胞在组织收缩方面的细胞生理学功能,是创伤愈合和瘢痕形成研究领域里一个新的和更为科学的方法。
Based on the monolayer cell culture system,we resuspended the scar-derived fibroblasts in a solution of polymerizing collagen to develop a“three-dimensional”cell culture system.In this sys- tem,cells,extracellular matrices and various modulating factors were associated to form an organic enti- ty,in which the fibroblasts express their biologic characteristics similar to that in living body.With the three-dimensional system,we could study not only the morphology,growth,proliferation and metabolism of fibroblasts,but also their cytophysiology,such as the role on connective tissue contrac- tion.We recommend this culture system as a better model in the research of wound healing and scar for- mation.
