幽门螺杆菌及其CagA基因感染与胃癌关系的研究

Study of the relationship between the Helicobacter pylori and CagA gene in patients with gastric carcinomaG2

目的:通过检测胃癌和浅表性胃炎组织幽门螺杆菌(Helicobacterpylori,Hp)和细胞毒素相关蛋白基因(CagA),探讨Hp及CagA基因与胃癌相关性及Hp形成胃癌的可能机制。方法:应用快速尿素酶试验和组织切片革兰染色和血清HpCagA抗体检测Hp,用PCR检测HpCagA基因。结果:慢性浅表性胃炎、胃癌组织中Hp检出率分别为45.9%和54.8%,两者差异无显著性(P>0.05),两种组织中HpCagA检出率分别为35.3%和77.5%,两者差异有显著性(P<0.005)。结论:胃癌组织与浅表性胃炎组Hp感染相比差异无显著性,胃癌组织中HpCagA基因检出率明显高于浅表性胃炎组,HpCagA基因与胃癌有一定相关性,HpCagA基因可能涉及胃癌的形成机制。

Objective： To investigate the relationship between the expression of Helicobacter pylori （Hp）, cytotoxic-associated gene A （CagA） and gastric cancer（CA2）,as well as the possible mechanism of Hp infection leading to oncogenesis by examining the Hp and CagA of patients with GC and chronic superficial gastritis（CSG）, Methods： Hp was examined by rapid urease test, gram stain and serum CagA antibodies. CagA gene of Hp was tested by polymerase chain reaction（PCR）. Results： There was no difference in the positive rates of Hp between CSG and GC（45.9 % vs 54.8 %, P 〉 0.05）, but the difference was significant in the positive rates of CagA gene between them （ 35.3 % vs 77.5 %, P 〈 0. 005）. Gonclusions： The positive rate of CagA gene in the GC group is significantly higher than that in the CSG group. Hp is correlated to CagA gene and GC. Hp CagA may be involved in the formation of gastric cancer.